The new species Bothriurus jesuita is described. This species belongs to the bonariensis species group and is closely related to Bothriurus chacoensis Maury & Acosta 1993 from which it can be distinguished by its thicker and taller chela and because of it is almost 30% larger. It can be distinguished from Bothriurus bonariensis (C.L. Koch 1842) because it has an apical filament on the basal lobe of the right hemispermatophore and because the frontal ridge reaches the frontal fold. Bothriurus jesuita has been collected in the northern region of Corrientes Province and in Misiones Province in an area that belongs to the “Paranaense” Phytogeographic Province.